{"id":4930,"date":"2021-03-20T18:39:58","date_gmt":"2021-03-20T17:39:58","guid":{"rendered":"https:\/\/meddists.com\/learn\/pre-clinical\/molecular-biology\/molecular-method-for-detection-of-proteins\/isoelectric-point-focusing\/"},"modified":"2021-03-24T10:48:59","modified_gmt":"2021-03-24T09:48:59","slug":"isoelectric-point-focusing","status":"publish","type":"page","link":"https:\/\/meddists.com\/learn\/pre-clinical\/molecular-biology\/molecular-method-for-detection-of-proteins\/isoelectric-point-focusing\/","title":{"rendered":"Isoelectric point focusing"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><div class=\"intro\">The isoelectris point (pI) is the pH at which a molecule has no electrical charge or is electrically neutral. <\/div><\/p>\n\n\n<span class=\"block-heading\" id=\"header_1\">\n<h2 class=\"wp-block-heading\" class=\"wp-block-heading\" class=\"title_collection title1\"><strong>ISOELECTRIC POINT \u2013 pI \u2013 ISOELECTRIC FOCUSING<\/strong><\/h2>\n<\/span><span class=\"block-content\" id=\"contents_1\">\n\n\n<p class=\"wp-block-paragraph\">The isoelectric point (pI) is the pH at which a molecule has no electrical charge or is electrically neutral. The net charge on the molecule is affected by the<sup> <\/sup>pH of its surroundings and can become more positively or negatively charged due to protons (H<sub>+<\/sub>), when the surface charge-determining ions are H<sup>+<\/sup>\/OH<sup>\u2212<\/sup>, the net surface charge is affected by the pH of the liquid in which the solid is submerged (Figure 1).<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"363\" src=\"https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO-1024x363.png\" alt=\"\" class=\"wp-image-4970\" srcset=\"https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO-1024x363.png 1024w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO-300x106.png 300w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO-768x273.png 768w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO.png 1364w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption><strong>Figure 1. I<\/strong>n the sample, we have several different proteins. The net charge of the different proteins determined by their amino acid composition. In these cases, we have various set of proteins with various charges. However, we know that every protein has a pH value where its charge will be zero. This is the isoelectric point. The numbers are representing these Isoelectric points, in a mixture.<\/figcaption><\/figure>\n\n\n<\/span><span class=\"block-heading\" id=\"header_2\">\n<h2 class=\"wp-block-heading\" class=\"wp-block-heading\" class=\"title_collection title1\">ISOELECTRIC FOCUSING (IEF)<\/h2>\n<\/span><span class=\"block-content\" id=\"contents_2\">\n\n\n<p class=\"wp-block-paragraph\">Isoelectric focusing (IEF), is a technique for separating different molecules by differences in their isoelectric point. By changing the pH the different pI molecules are reorganized based on their net-zero charge\/pH levels. By changing the pH the different pI molecules are reorganized based on their net-zero charge\/pH levels. The method is used in a prefiltering of proteins, in PROTEOMIC analyses (Figure 2).<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"435\" src=\"https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO2-1024x435.png\" alt=\"\" class=\"wp-image-4973\" srcset=\"https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO2-1024x435.png 1024w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO2-300x127.png 300w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO2-768x326.png 768w, https:\/\/meddists.com\/learn\/wp-content\/uploads\/2021\/03\/ISO2.png 1133w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption><strong>Figure 2. EIF.<\/strong> After the pH rearrangement the molecules are separated by their Ip.<\/figcaption><\/figure>\n<\/span><div id=\"the_titles\" style=\"display:none;\"><h2 class=\"wp-block-heading\" class=\"wp-block-heading\"><strong>ISOELECTRIC POINT \u2013 pI \u2013 ISOELECTRIC FOCUSING<\/strong><\/h2><h2 class=\"wp-block-heading\" class=\"wp-block-heading\">ISOELECTRIC FOCUSING (IEF)<\/h2><\/div>","protected":false},"excerpt":{"rendered":"<p>ISOELECTRIC POINT \u2013 pI \u2013 ISOELECTRIC FOCUSING The isoelectric point (pI) is the pH at which a molecule has no electrical charge or is electrically neutral. The net charge on the molecule is affected by the pH of its surroundings and can become more positively or negatively charged due to protons (H+), when the surface [&hellip;]<\/p>\n","protected":false},"author":23,"featured_media":0,"parent":2308,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4930","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Isoelectric point focusing &#8211; Meddists<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/meddists.com\/learn\/pre-clinical\/molecular-biology\/molecular-method-for-detection-of-proteins\/isoelectric-point-focusing\/\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/meddists.com\\\/learn\\\/pre-clinical\\\/molecular-biology\\\/molecular-method-for-detection-of-proteins\\\/isoelectric-point-focusing\\\/\",\"url\":\"https:\\\/\\\/meddists.com\\\/learn\\\/pre-clinical\\\/molecular-biology\\\/molecular-method-for-detection-of-proteins\\\/isoelectric-point-focusing\\\/\",\"name\":\"Isoelectric point focusing &#8211; 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