The isoelectris point (pI) is the pH at which a molecule has no electrical charge or is electrically neutral.
ISOELECTRIC POINT – pI – ISOELECTRIC FOCUSING
The isoelectric point (pI) is the pH at which a molecule has no electrical charge or is electrically neutral. The net charge on the molecule is affected by the pH of its surroundings and can become more positively or negatively charged due to protons (H+), when the surface charge-determining ions are H+/OH−, the net surface charge is affected by the